7. Peotone Glucose Yeast extract Medium PGY (蛋白胨、酵母膏、葡萄糖培养基)
Peptone(蛋白胨) 10g Yeast extract (酵母膏) 5g
Glucose (葡萄糖) 1g Distilled water (蒸馏水) 1L
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8. Glycerol Agar (甘油琼脂)
Peptone (蛋白胨) 5g Beef extract (酵母膏) 3g
Glycerol (甘油) 20g Top water (自来水) 1000ml
Agar (琼脂) 15g pH 7.0-7.2
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9. Rhizobium medium (根瘤菌培养基)AS 9
Yeast eztract (酵母膏) 1g Soil eztract (土壤浸提液) 200ml
Mannitol (甘露醇) 10g Agar (琼脂) 15g
Distilled water (蒸馏水) 800ml pH 7.2
[Note]:Soil extract:Suspend 50g finely and dried gardon soil in 200ml of tap water. Autoclave at 121℃ for 1 hr. Decant through cotton-cloth, filler though paper, make up volume to 200ml. Resterilize for 20 minutes at 121℃ , then mixed with othringredients and distributed. (土壤浸提液的制法:取土壤50克,加水200毫升,15磅蒸煮1小时,经滤纸过滤后加水补足到200毫升。)
适用范围:大豆根瘤菌(慢生型)、豇豆慢生根瘤菌、花生根瘤菌、紫云英根瘤菌、
大豆根瘤菌(快生型)、大豆根瘤菌、豌豆根瘤菌、苜蓿根瘤菌、田菁根瘤菌
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10. Mannitol Agar (甘露醇琼脂)
Yeast extract (酵母膏) 5g Peptone (蛋白胨) 3g
Mannitol (甘露醇) 25g Agar (琼脂) 15g
Distilled water (蒸馏水) 1000ml
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11. Glucose Asparagine (葡萄糖、天门冬素琼脂)
Glucose (葡萄糖) 10g Asparagine (天门冬素) 0.5g
K2HPO4 0.5g Water (水) 1000ml
pH 7.2-7.4
适用范围:刺孢小单孢菌绛红变种、紫色小单孢菌(绛红小单孢菌)
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12. Gause′s Synthetic Agar (高氏合成一号琼脂)
KNO3 1g Soluble starch(可溶性淀粉) 20g
K2HPO4 0.5g MgSO4.7H2O 0.5g
NaCl 0.5g FeSO4 0.01g
Agar (琼脂) 20g water (水) 1000ml
pH 7.2-7.4
适用范围:刺孢小单孢菌绛红变种、紫色小单孢菌(绛红小单孢菌)、白黄链霉菌、白色链霉菌、抗生链霉菌、双重轮丝链霉菌、产色链霉菌、烬灰链霉菌、天蓝色链霉菌、灭蚊链霉菌、红霉素链霉菌、青色链霉菌、球孢链霉菌、浅灰链霉菌、灰色链霉菌、吸水链霉菌、淡紫灰链霉菌、黄色长孢链霉菌、藤黄色链霉菌、细黄链霉菌、黑化链霉菌、玫瑰色链霉菌、华美链霉菌、嗜热链霉菌、委内瑞拉链霉菌、紫色直丝链霉菌、紫色链霉菌、绿色链霉菌
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